Classification and cladistics: exam questions

7 questions, 22 marks. Write your answers on paper, then open each mark scheme.

Spec A3.2
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Questions and mark scheme (PDF)5 pages

Question 1

Paper 1A style

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What does a node on a cladogram represent?

  1. A species alive today
  2. A hypothetical common ancestor
  3. A mutation in a single base
  4. The time since a species became extinct
[1 mark]
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Answer: B [1]

Question 2

Paper 1A style

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Which evidence led to the classification of all organisms into three domains?

  1. Comparisons of cell wall structure
  2. Base sequences of ribosomal RNA
  3. Numbers of chromosomes
  4. Fossils of the earliest cells
[1 mark]
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Answer: B [1]

Question 3

Paper 1A style

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Why can the molecular clock only give an estimate of when two clades diverged?

  1. Sequence differences stop accumulating once two clades have diverged.
  2. Only amino acid sequences, not base sequences, can be used.
  3. Mutation rates are affected by generation time, population size and selective pressure.
  4. The number of sequence differences decreases as time passes.
[1 mark]
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Answer: C [1]

Question 4

Paper 2A style

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(a) Outline the need for classification of organisms. [2] (b) Explain one advantage of classifying organisms according to evolutionary relationships. [2]

[4 marks]
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  • (a) immense diversity of species / very large number of species [1]
  • (a) classification makes diversity manageable / allows identification [1]
  • (a) after classification, further study is facilitated / information about related species can be found [1]
  • (b) all members of the group have evolved from a common ancestor [1]
  • (b) characteristics of members can be predicted [1]
  • (b) because characteristics are shared within a clade [1]
  • (b) e.g. a species related to one that produces a useful drug may produce it too [1]
  • max 4

Question 5

Paper 1B style

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The table shows the number of amino acid differences in a protein between four species, P, Q, R and S (practice data). (a) Identify the two most closely related species. [1] (b) Assuming differences accumulate at 1 difference per 10 million years, estimate when P and S diverged. [1] (c) Deduce the sequence in which the species diverged, and describe the most probable cladogram. [2] (d) Suggest why the estimate in (b) may be inaccurate. [1]

Amino acid differences between species
PQRS
P–91012
Q9–38
R103–7
S1287–
[5 marks]
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  • (a) Q and R [1]
  • (b) 12 × 10 = 120 million years ago [1]
  • (c) P diverged first / P is the outgroup [1]
  • (c) then S; Q and R share the most recent common ancestor [1]
  • (c) cladogram: P branches from the root, then S, with Q and R as sister species at the last node [1]
  • (d) mutation rate may not be constant / varies with generation time / population size / selective pressure [1] Accept: P–Q differences (9) give a different date (90 million years) for the same divergence, so rates differ between lineages
  • max 5

Question 6

Paper 2A style

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A cladogram shows a root leading to node 1, which splits into species A and node 2. Node 2 splits into species B and node 3. Node 3 splits into species C and species D. (a) State which node represents the most recent common ancestor of B and D. [1] (b) Deduce how many clades contain species B. [1] (c) Identify the species most closely related to C. [1]

[3 marks]
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  • (a) node 2 [1]
  • (b) two (the clade from node 1 and the clade from node 2) [1] Accept three if B alone is counted as a clade
  • (c) D [1]

Question 7

Paper 2B style

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Discuss how cladistics has changed the classification of organisms.

[7 marks]
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  • traditional hierarchy of kingdom, phylum, class, order, family, genus, species [1]
  • fixed ranks are arbitrary / do not reflect gradation of variation / do not always match patterns of divergence [1]
  • cladistics uses unranked clades: an ancestor and all its descendants [1]
  • evidence from base sequences of genes / amino acid sequences of proteins [1]
  • sequence evidence is more objective than morphology [1]
  • morphology can mislead because of convergent evolution [1]
  • cladograms built using parsimony / fewest sequence changes [1]
  • traditional groups found not to be clades were reclassified [1]
  • e.g. figwort family / Scrophulariaceae: many species moved to other families [1]
  • e.g. birds lie within the reptiles, so the class Reptilia is not a clade [1]
  • rRNA base sequences led to three domains: Bacteria, Archaea, Eukarya (1977) [1]
  • a new level above kingdoms / prokaryotes split into two domains [1]
  • paradigm shift / earlier classifications falsified [1]
  • but cladograms are hypotheses; different criteria / data can give different trees [1]
  • max 7