Photosynthesis: energy, carbon dioxide, water and oxygen

Molecules (Interaction and interdependence) · Photosynthesis · note 1 of 9

Photosynthesis: energy, carbon dioxide, water and oxygenSpec C1.3.1, C1.3.2, C1.3.3

In short

Photosynthesis transforms light energy into chemical energy when carbon compounds are produced. Carbon dioxide is converted to glucose using hydrogen obtained by splitting water: carbon dioxide + water → glucose + oxygen. The oxygen comes from the splitting of water and is a by-product released by plants, algae and cyanobacteria. This supplies most of the chemical energy in ecosystems.

Photosynthesis involves the transformation of light energy to chemical energy. Light energy absorbed by pigments is used to make carbon compounds, and the energy is then held in the chemical bonds of those compounds. This energy transformation supplies most of the chemical energy needed for life processes in ecosystems: producers use it directly, and consumers and decomposers obtain it by feeding.

Carbon dioxide, water and glucose

Carbon dioxide is converted to glucose using hydrogen obtained by splitting water. Carbon dioxide provides the carbon and oxygen atoms of glucose, but glucose also contains hydrogen, which must come from somewhere: it comes from water molecules, split using energy from light.

carbon dioxide + water → glucose + oxygen

Oxygen as a by-product

When water is split, the hydrogen is used and the oxygen is released as a by-product. So the oxygen produced by photosynthesis comes from the splitting of water, not from carbon dioxide. Oxygen-producing photosynthesis happens in plants, algae and cyanobacteria.

Common mistake:

Do not write that the oxygen released comes from carbon dioxide. It comes from water; the oxygen atoms of CO₂ end up in glucose and water.

Exam tip:

Linking question: the consequences of photosynthesis for ecosystems include supplying chemical energy to food chains and removing carbon dioxide from the atmosphere.

Written and checked against the IB Biology HL specification · Updated October 2026

Frequently asked questions

Why do plants absorb red and blue light but not green?

Chlorophyll absorbs mainly red and blue light because only photons with energy matching the gaps between its electron energy levels can excite its electrons. Green light does not match well, so most of it is reflected or transmitted, which is why leaves look green. Accessory pigments such as carotenoids absorb some other wavelengths.

What is the difference between an absorption spectrum and an action spectrum?

An absorption spectrum shows the percentage of light a pigment absorbs at each wavelength, while an action spectrum shows the rate of photosynthesis at each wavelength. Both peak in blue and red light. The action spectrum stays above zero in green light because accessory pigments absorb some of it, and the similar shapes show absorbed light drives photosynthesis.

How do you calculate Rf values in chromatography?

Divide the distance moved by the pigment by the distance moved by the solvent front, both measured from the origin line. Rf values lie between 0 and 1 and have no units. Pigments are identified by their colour and by comparing their Rf values with reference values for the same solvent and medium.

All 5 questions on Photosynthesis